翻訳と辞書 Words near each other ・ Overnight Sensational ・ Overnight Success ・ Overnight Success (Dave Dobbyn album) ・ Overnight Success (Neil Sedaka album) ・ Overnight Success (song) ・ Overlander Falls ・ Overlander Roadhouse ・ Overlanders ・ Overlanders, Edmonton ・ Overlanding ・ Overlangbroek ・ Overlap ・ Overlap (railway signalling) ・ Overlap (term rewriting) ・ Overlap coefficient ・ Overlap extension polymerase chain reaction ・ Overlap syndrome ・ Overlap zone ・ Overlapped I/O ・ Overlapping consensus ・ Overlapping distribution method ・ Overlapping gene ・ Overlapping generations ・ Overlapping generations model ・ Overlapping interval topology ・ Overlapping markup ・ Overlapping subproblems ・ Overlap–add method ・ Overlap–save method ・ Overlawyered Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Overlap extension polymerase chain reaction ： ウィキペディア英語版 Overlap extension polymerase chain reaction ::''This page assumes familiarity with the terms and components used in the polymerase chain reaction (PCR) process.'' The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide. ==Splicing of DNA Molecules== As in most PCR reactions, two primers -one for each end- are used per sequence. To splice two DNA molecules, special primers are used at the ends that are to be joined. For each molecule, the primer at the end to be joined is constructed such that it has a 5' overhang complementary to the end of the other molecule. Following annealing when replication occurs, the DNA is extended by a new sequence that is complementary to the molecule it is to be joined to. Once both DNA molecules are extended in such a manner, they are mixed and a PCR is carried out with only the primers for the far ends. The overlapping complementary sequences introduced will serve as primers and the two sequences will be fused. This method has an advantage over other gene splicing techniques in not requiring restriction sites. To get higher yields, some primers are used in excess as in asymmetric PCR. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Overlap extension polymerase chain reaction」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.